Native MS Guided Structural Biology Center

De Novo Design of Transmembrane β-Barrels

Vorobieva, A. A.; White, P.; Liang, B.; Horne, J. E.; Bera, A. K.; Chow, C. M.; Gerben, S.; Marx, S.; Kang, A.; Stiving, A. Q.; Harvey, S. R.; Marx, D. C.; Khan, G. N.; Fleming, K. G.; Wysocki, V. H.; Brockwell, D. J.; Tamm, L. K.; Radford, S. E.; Baker, D.

Science, 2021, 371 (6531), eabc8182, DOI: 10.1126/science.abc8182

A metabolic intermediate of the fructose-asparagine utilization pathway inhibits growth of a Salmonella fraB mutan.

Sabag-Daigle, A.; Blunk, H. M.; Sengupta, A.; Wu, J.; Bogard, A. J.; Ali, M. M.; Stahl, C.; Wysocki, V. H.; Gopalan,V.; Behrman, E. J.; Ahmer, B. M. M.

Scientific Reports. 2016; DOI: 10.1038/srep28117. Request reprint © (pdf)

Foldability of a Natural De Novo Evolved Protein.

Bungard, D.; Copple, J. S.; Yan, J.; Chhun, J. J.; Kumirov, V. K.; Foy, S. G.; Masel, J.; Wysocki, V. H.; Cordes, M. H. J.

Structure. 2017; 25: 1-10. Request reprint © (pdf)

Native IM-Orbitrap MS: Resolving what was hidden.

Poltash, M.L.; McCabe, J.W.; Shirzadeh, M.; Laganowsky,; Russell, D.H.

Trends in Analytical Chemistry, 2020, 124:115533. DOI: https://doi.org/10.1016/j.trac.2019.05.035

Surface-Induced Dissociation: An Effective Method for Characterization of Protein Quaternary Structure.

Stiving, A. Q.; VanAernum Z.; Busch, F.; Harvey S. R.; Sarni, S.; Wysocki, V. H.

Anal Chem., 2019, 91, 190-209. DOI:10.1021/acs.analchem.8b05071.

Dissecting the Large Noncovalent Protein Complex GroEL with Surface-Induced Dissociation and Ion Mobility-Mass Spectrometry.

Zhou, M.; Jones, C.M.; Wysocki, V.H.

Anal Chem. 2013; 85(17):8262-7. Request reprint © (pdf)

Tunable hetero-assembly of a plant pseudoenzyme-enzyme complex.

Novikova I. V.; Zhou M.; Du C.; Parra M., Kim D. N.; VanAernum Z. L.; Shaw J. B.; Hellmann H.; Wysocki V. H.; Evans J. E.

ACS Chem. Biol. 2021, 16 (11), 2315–2325. https://doi.org/10.1021/acschembio.1c00475.

Refining the Structural Model of a Hetero-hexameric Protein Complex: Surface Induced Dissociation and Ion Mobility Provide Key Connectivity and Topology Information.

Song, Y.; Nelp, M. T.; Bandarian, V.; Wysocki, V. H.

ACS Central Science. 2015; 1, 477-487.

Comparative Structural Analysis of 20S Proteasome Ortholog Protein Complexes by Native Mass Spectrometry.

Vimer, S.; Ben-Nissan, G.; Morgenstern, D.; Kumar-Deshmukh, K.; Polkinghorn, C.; Quintyn R.S.; Vsil’ev, Y.V.; Beckman, J.S.; Elad, N.; Wysocki, V.H.; Sharon, M.

ACS Cent. Sci. 2020, 6, 573-588. DOI:10.1021/acscentsci.0c00080

Predicting Protein Complex Structure from Surface-Induced Dissociation Mass Spectrometry Data.

Seffernick, J. T.; Harvey, S. R.; Wysocki, V. H.; Lindert, S.

ACS Cent. Sci. 2019, 5 (8), 1330–1341. https://doi.org/10.1021/acscentsci.8b00912.

Native MS Guided Structural Biology Center

Georgia Institute of Technology