We have received notice of award of a 6.52M RM1 grant for our next five year period, converting the Biomedical Technology Research Resource (BTRR) to a national Biomedical Technology Optimization and Dissemination (BTOD) Center. The overarching goal of the Native MS Guided Structural Biology Center is to translate an integrated native mass spectrometry (nMS) workflow for characterization of macromolecular protein and nucleoprotein complexes to the biomedical research community. The tools provided for expert and non-expert users will allow them to integrate nMS approaches in synergy with other structural biology tools, taking advantage of nMS from project beginning to end, with dissemination through vendor partnerships and training. The Center works with investigators across the nation and globe on challenging biomedical projects ranging from HIV to anti-coronavirus immunogens and neurological disorders.