A Workshop in Native Mass Spectrometry will be held on July 23, 2023 in conjunction with AMS 2023 to be held in Austin, Texas. Instructors are Vicki Wysocki (OSU), Dave Russell (Texas A&M University), Art Laganowsky (Texas A&M), Brandon Ruotolo (University of Michigan). 

Introduction:

Native mass spectrometry is an exploding area in the mass spectrometry community. Native MS is making significant contributions to structural biology and because of its ease of application and broad applicability, can lead and/or guide an integrated approach that also uses other structural biology tools. This course is designed to introduce the broad field to new practitioners of native MS.

Topics to be covered include:

• intro and case studies for protein:protein, protein ligand, RNA: protein, DNA:protein, and membrane protein complexes

• sample preparation for native MS

• instrumentation used for native MS -  how does it differ from other MS, activation methods

• spray conditions to keep soluble complexes native

• spray conditions for membrane protein complexes (micelles, liposomes, nanodiscs)

• ion mobility for native MS

Prerequisites: Basic knowledge of mass spectrometry, including some familiarity with ESI and mass analyzers