A two-day short course will be presented on June 3-4, 2023 in Houston, TX, in conjunction with the annual American Society for Mass Spectrometry Conference. The course is designed to introduce the broad field of native mass spectrometry to new practitioners of native MS. Instructors are David Russell, Arthur Laganowsky, Brandon Ruotolo and Sophie Harvey. Topics include: Sample preparation for native MS; Membrane protein native MS; Instrumentation for nMS; Instrument conditions to keep soluble complexes native; Online separations approaches couples to nMS; Activation methods for nMS (non-covalent); Native-omics; Ion mobility instrumentation for nMS; Theoretical calculations and software for CCS determination; Intro to software tools and examples; Software tools demonstration; Variable temperature ESI; Thermodynamics and binding constants; Integrated platform and case studies. Please visit the ASMS website for more information.